Coding

Part:BBa_K896988:Design

Designed by: Hsieh Tien-Chan   Group: iGEM12_NYMU-Taipei   (2012-04-26)


Homo sapiens solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2076
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 671
    Illegal BamHI site found at 1997
    Illegal XhoI site found at 159
    Illegal XhoI site found at 3184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 389
    Illegal NgoMIV site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2980
    Illegal SapI site found at 840
    Illegal SapI.rc site found at 217


Design Notes

In vitro DNase I footprinting, electrophoretic mobility shift assays, and reporter gene assays identified functional AP-2 and Sp1 sites in this region. The KCC1 promoter was transactivated by forced expression of AP-2 in heterologous cells. Sequences encoding the InR, DPE, AP-2, and Sp1 sites were 100% conserved between human and murine KCC1 genes. In vivo studies using chromatin immunoprecipitation assays with antihistone H3 and antihistone H4 antibodies demonstrated hyperacetylation of this core promoter region. (Blood. 2004;103:4302-4309)


Source

reference: Human potassium chloride cotransporter 1 (SLC12A4) promoter is regulated by AP-2 and contains a functional downstream promoter element Guo-Ping Zhou, Clara Wong, Robert Su, Scott C. Crable, Kathleen P. Anderson, and Patrick G. Gallagher

References